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    Mendeley Ltd original western blot images
    Purine metabolism plays a crucial role in ovarian and cervical cancer progression (A) Expression of purine nucleoside phosphorylase ( PNP ) in normal cervical tissues and CC tissues; normal, n = 13; tumor, n = 306. (B) Relative PNP RNA level in CaSki cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. TBP was used as the internal reference gene. Statistical analysis of PNP RNA level between the 4 groups. (C–E) Relative Ki67 , PCNA , and CDK1 RNA levels in CaSki cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (F) Expression of PNP in normal ovarian tissues and ovarian cancer tissues; normal, n = 88; tumor, n = 427. (G) Relative PNP RNA level in PA1 cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. (H–J) Relative Ki67 , PCNA , and CDK1 RNA levels in PA1 cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (K and L) The knockdown efficiency of PNP in CaSki and PA1 cells, verified via <t>western</t> <t>blot</t> assay. (M and N) Detection of cell proliferation in CaSki (left) and PA1 (right) cells with stable PNP knockdown via MTT assay at 24, 48, and 72 h. (O) Detection of foci formation in CaSki and PA1 cells with stable PNP knockdown via colony formation assay. Representative colony formation <t>images</t> of CaSki and PA1 cells in PNP stable knockdown groups (shPNP #1 and shPNP #2) and the MOCK control group. (P) Quantitative statistical analysis of colony formation number in CaSki and PA1 cells among the three groups. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, determined by one-way ANOVA (B–E, G–J, M, N, and P) and Wilcoxon rank-sum test (A and F). See also .
    Original Western Blot Images, supplied by Mendeley Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    1) Product Images from "Uncovering metabolic reprogramming in ovarian and cervical cancers with multi-omics"

    Article Title: Uncovering metabolic reprogramming in ovarian and cervical cancers with multi-omics

    Journal: iScience

    doi: 10.1016/j.isci.2026.115834

    Purine metabolism plays a crucial role in ovarian and cervical cancer progression (A) Expression of purine nucleoside phosphorylase ( PNP ) in normal cervical tissues and CC tissues; normal, n = 13; tumor, n = 306. (B) Relative PNP RNA level in CaSki cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. TBP was used as the internal reference gene. Statistical analysis of PNP RNA level between the 4 groups. (C–E) Relative Ki67 , PCNA , and CDK1 RNA levels in CaSki cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (F) Expression of PNP in normal ovarian tissues and ovarian cancer tissues; normal, n = 88; tumor, n = 427. (G) Relative PNP RNA level in PA1 cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. (H–J) Relative Ki67 , PCNA , and CDK1 RNA levels in PA1 cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (K and L) The knockdown efficiency of PNP in CaSki and PA1 cells, verified via western blot assay. (M and N) Detection of cell proliferation in CaSki (left) and PA1 (right) cells with stable PNP knockdown via MTT assay at 24, 48, and 72 h. (O) Detection of foci formation in CaSki and PA1 cells with stable PNP knockdown via colony formation assay. Representative colony formation images of CaSki and PA1 cells in PNP stable knockdown groups (shPNP #1 and shPNP #2) and the MOCK control group. (P) Quantitative statistical analysis of colony formation number in CaSki and PA1 cells among the three groups. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, determined by one-way ANOVA (B–E, G–J, M, N, and P) and Wilcoxon rank-sum test (A and F). See also .
    Figure Legend Snippet: Purine metabolism plays a crucial role in ovarian and cervical cancer progression (A) Expression of purine nucleoside phosphorylase ( PNP ) in normal cervical tissues and CC tissues; normal, n = 13; tumor, n = 306. (B) Relative PNP RNA level in CaSki cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. TBP was used as the internal reference gene. Statistical analysis of PNP RNA level between the 4 groups. (C–E) Relative Ki67 , PCNA , and CDK1 RNA levels in CaSki cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (F) Expression of PNP in normal ovarian tissues and ovarian cancer tissues; normal, n = 88; tumor, n = 427. (G) Relative PNP RNA level in PA1 cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. (H–J) Relative Ki67 , PCNA , and CDK1 RNA levels in PA1 cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (K and L) The knockdown efficiency of PNP in CaSki and PA1 cells, verified via western blot assay. (M and N) Detection of cell proliferation in CaSki (left) and PA1 (right) cells with stable PNP knockdown via MTT assay at 24, 48, and 72 h. (O) Detection of foci formation in CaSki and PA1 cells with stable PNP knockdown via colony formation assay. Representative colony formation images of CaSki and PA1 cells in PNP stable knockdown groups (shPNP #1 and shPNP #2) and the MOCK control group. (P) Quantitative statistical analysis of colony formation number in CaSki and PA1 cells among the three groups. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, determined by one-way ANOVA (B–E, G–J, M, N, and P) and Wilcoxon rank-sum test (A and F). See also .

    Techniques Used: Expressing, Knockdown, Control, Quantitative RT-PCR, Western Blot, MTT Assay, Colony Assay



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    original western blot images  (Mendeley Ltd)
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    Mendeley Ltd original western blot images
    Purine metabolism plays a crucial role in ovarian and cervical cancer progression (A) Expression of purine nucleoside phosphorylase ( PNP ) in normal cervical tissues and CC tissues; normal, n = 13; tumor, n = 306. (B) Relative PNP RNA level in CaSki cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. TBP was used as the internal reference gene. Statistical analysis of PNP RNA level between the 4 groups. (C–E) Relative Ki67 , PCNA , and CDK1 RNA levels in CaSki cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (F) Expression of PNP in normal ovarian tissues and ovarian cancer tissues; normal, n = 88; tumor, n = 427. (G) Relative PNP RNA level in PA1 cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. (H–J) Relative Ki67 , PCNA , and CDK1 RNA levels in PA1 cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (K and L) The knockdown efficiency of PNP in CaSki and PA1 cells, verified via <t>western</t> <t>blot</t> assay. (M and N) Detection of cell proliferation in CaSki (left) and PA1 (right) cells with stable PNP knockdown via MTT assay at 24, 48, and 72 h. (O) Detection of foci formation in CaSki and PA1 cells with stable PNP knockdown via colony formation assay. Representative colony formation <t>images</t> of CaSki and PA1 cells in PNP stable knockdown groups (shPNP #1 and shPNP #2) and the MOCK control group. (P) Quantitative statistical analysis of colony formation number in CaSki and PA1 cells among the three groups. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, determined by one-way ANOVA (B–E, G–J, M, N, and P) and Wilcoxon rank-sum test (A and F). See also .
    Original Western Blot Images, supplied by Mendeley Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/original western blot images/product/Mendeley Ltd
    Average 86 stars, based on 1 article reviews
    original western blot images - by Bioz Stars, 2026-06
    86/100 stars
    		  Buy from Supplier

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    Purine metabolism plays a crucial role in ovarian and cervical cancer progression (A) Expression of purine nucleoside phosphorylase ( PNP ) in normal cervical tissues and CC tissues; normal, n = 13; tumor, n = 306. (B) Relative PNP RNA level in CaSki cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. TBP was used as the internal reference gene. Statistical analysis of PNP RNA level between the 4 groups. (C–E) Relative Ki67 , PCNA , and CDK1 RNA levels in CaSki cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (F) Expression of PNP in normal ovarian tissues and ovarian cancer tissues; normal, n = 88; tumor, n = 427. (G) Relative PNP RNA level in PA1 cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. (H–J) Relative Ki67 , PCNA , and CDK1 RNA levels in PA1 cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (K and L) The knockdown efficiency of PNP in CaSki and PA1 cells, verified via western blot assay. (M and N) Detection of cell proliferation in CaSki (left) and PA1 (right) cells with stable PNP knockdown via MTT assay at 24, 48, and 72 h. (O) Detection of foci formation in CaSki and PA1 cells with stable PNP knockdown via colony formation assay. Representative colony formation images of CaSki and PA1 cells in PNP stable knockdown groups (shPNP #1 and shPNP #2) and the MOCK control group. (P) Quantitative statistical analysis of colony formation number in CaSki and PA1 cells among the three groups. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, determined by one-way ANOVA (B–E, G–J, M, N, and P) and Wilcoxon rank-sum test (A and F). See also .

    Journal: iScience

    Article Title: Uncovering metabolic reprogramming in ovarian and cervical cancers with multi-omics

    doi: 10.1016/j.isci.2026.115834

    Figure Lengend Snippet: Purine metabolism plays a crucial role in ovarian and cervical cancer progression (A) Expression of purine nucleoside phosphorylase ( PNP ) in normal cervical tissues and CC tissues; normal, n = 13; tumor, n = 306. (B) Relative PNP RNA level in CaSki cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. TBP was used as the internal reference gene. Statistical analysis of PNP RNA level between the 4 groups. (C–E) Relative Ki67 , PCNA , and CDK1 RNA levels in CaSki cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (F) Expression of PNP in normal ovarian tissues and ovarian cancer tissues; normal, n = 88; tumor, n = 427. (G) Relative PNP RNA level in PA1 cells after PNP knockdown (shPNP #1/2/3 group) and control group (MOCK group) detected by RT-qPCR. (H–J) Relative Ki67 , PCNA , and CDK1 RNA levels in PA1 cells of PNP stable knockdown groups (shPNP #1 and shPNP #2) and MOCK control group detected by RT-qPCR. (K and L) The knockdown efficiency of PNP in CaSki and PA1 cells, verified via western blot assay. (M and N) Detection of cell proliferation in CaSki (left) and PA1 (right) cells with stable PNP knockdown via MTT assay at 24, 48, and 72 h. (O) Detection of foci formation in CaSki and PA1 cells with stable PNP knockdown via colony formation assay. Representative colony formation images of CaSki and PA1 cells in PNP stable knockdown groups (shPNP #1 and shPNP #2) and the MOCK control group. (P) Quantitative statistical analysis of colony formation number in CaSki and PA1 cells among the three groups. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001, determined by one-way ANOVA (B–E, G–J, M, N, and P) and Wilcoxon rank-sum test (A and F). See also .

    Article Snippet: • Original western blot images have been deposited at Mendeley (Mendeley Data: https://data.mendeley.com/datasets/wv839nvbf8/1 ) and are publicly available.

    Techniques: Expressing, Knockdown, Control, Quantitative RT-PCR, Western Blot, MTT Assay, Colony Assay